A comparison of UV induced DNA photoproducts from isolated and non-isolated developing bacterial forespores
- PMID: 6409107
- DOI: 10.1016/0006-291x(83)91771-0
A comparison of UV induced DNA photoproducts from isolated and non-isolated developing bacterial forespores
Abstract
UV-induced photoproduct formation has been compared in non-isolated and isolated developing forespores. We have found that levels of spore type photoproducts are greatly affected by mother cell DNA. We have also observed the presence of the photoproduct 6-4'-(pyrimidin-2'-one)-thymine in developing forespores. We conclude from these and other data in the literature that the degree of hydration around the forespore DNA is reduced by the presence of dipicolinic acid which influences photoproduct formation without causing a change in conformational state.
Similar articles
-
High-pressure liquid chromatography assay for quantitatively monitoring spore photoproduct repair mediated by spore photoproduct lyase during germination of uv-irradiated Bacillus subtilis spores.Anal Biochem. 1994 Aug 15;221(1):61-5. doi: 10.1006/abio.1994.1379. Anal Biochem. 1994. PMID: 7985805
-
The ultraviolet photochemistry and photobiology of vegetative cells and spores of Bacillus megaterium.Biophys J. 1968 Jan;8(1):17-28. doi: 10.1016/S0006-3495(68)86471-9. Biophys J. 1968. PMID: 4966691 Free PMC article.
-
Photoreactivation, photoproduct formation, and deoxyribonucleic acid state in ultraviolet-irradiated sporulating cultures of Bacillus cereus.J Bacteriol. 1974 Oct;120(1):516-23. doi: 10.1128/jb.120.1.516-523.1974. J Bacteriol. 1974. PMID: 4214215 Free PMC article.
-
Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals.J Appl Microbiol. 2006 Sep;101(3):514-25. doi: 10.1111/j.1365-2672.2005.02736.x. J Appl Microbiol. 2006. PMID: 16907802 Review.
-
Resistance of spores of Bacillus species to ultraviolet light.Environ Mol Mutagen. 2001;38(2-3):97-104. doi: 10.1002/em.1058. Environ Mol Mutagen. 2001. PMID: 11746741 Review.
Cited by
-
Binding of DNA to alpha/beta-type small, acid-soluble proteins from spores of Bacillus or Clostridium species prevents formation of cytosine dimers, cytosine-thymine dimers, and bipyrimidine photoadducts after UV irradiation.J Bacteriol. 1992 May;174(9):2874-80. doi: 10.1128/jb.174.9.2874-2880.1992. J Bacteriol. 1992. PMID: 1569018 Free PMC article.
-
Desiccation tolerance of prokaryotes.Microbiol Rev. 1994 Dec;58(4):755-805. doi: 10.1128/mr.58.4.755-805.1994. Microbiol Rev. 1994. PMID: 7854254 Free PMC article. Review.
-
I will survive: protecting and repairing spore DNA.J Bacteriol. 1992 May;174(9):2737-41. doi: 10.1128/jb.174.9.2737-2741.1992. J Bacteriol. 1992. PMID: 1569005 Free PMC article. Review. No abstract available.
-
Resistance of Bacillus endospores to extreme terrestrial and extraterrestrial environments.Microbiol Mol Biol Rev. 2000 Sep;64(3):548-72. doi: 10.1128/MMBR.64.3.548-572.2000. Microbiol Mol Biol Rev. 2000. PMID: 10974126 Free PMC article. Review.
-
The two major spore DNA repair pathways, nucleotide excision repair and spore photoproduct lyase, are sufficient for the resistance of Bacillus subtilis spores to artificial UV-C and UV-B but not to solar radiation.Appl Environ Microbiol. 1996 Jul;62(7):2221-7. doi: 10.1128/aem.62.7.2221-2227.1996. Appl Environ Microbiol. 1996. PMID: 8779559 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources