Agar-embedded urinary stones: a technique useful for studying microscopic architecture

Abstract

A procedure for studying urinary stones by various microscopic techniques is described. The stones are sectioned into approximately 0.2 to 1.0 mm. thick pieces using a low-speed saw. The sections are then embedded in agar and decalcified using 0.25 M ethylenediaminetetracetic acid at pH 7.2. The decalcified residue is then processed for light microscopy and scanning and transmission electron microscopy as with any other biological tissue. The results indicate that the ethylenediaminetetracetic acid-insoluble stone matrix keeps its architectural integrity and can be studied like other biological materials.