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Comparative Study
. 1983 Sep;46(3):549-52.
doi: 10.1128/aem.46.3.549-552.1983.

Simple and rapid method for isolating large plasmid DNA from lactic streptococci

D G Anderson, L L McKay
Comparative Study

Simple and rapid method for isolating large plasmid DNA from lactic streptococci

D G Anderson et al. Appl Environ Microbiol. 1983 Sep.

Abstract

A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning, or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.

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References

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