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. 1983 Nov;80(22):6957-61.
doi: 10.1073/pnas.80.22.6957.

Characterization of the human receptor for T-cell growth factor

W J Leonard, J M Depper, R J Robb, T A Waldmann, W C Greene

Characterization of the human receptor for T-cell growth factor

W J Leonard et al. Proc Natl Acad Sci U S A. 1983 Nov.

Abstract

Anti-Tac monoclonal antibody has been identified as a putative antibody against the receptor for T-cell growth factor (TCGF). We now show that: (i) TCGF blocks 85% of 3H-labeled anti-Tac binding to phytohemagglutinin-activated lymphoblasts and (ii) both anti-Tac and anti-TCGF immunoprecipitate a protein band that appears to represent TCGF crosslinked to its receptor on HUT-102B2 cells. In HUT-102B2 cells, the TCGF receptor is a Mr 50,000 glycoprotein with internal disulfide bond(s) and a pI of 5.5-6.0, and it represents approximately equal to 0.05% of total cellular de novo protein synthesis. It contains a peptide of Mr 33,000 that is processed to a mature form that includes N-linked and O-linked sugars and sialic acid.

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