Tannic acid improves the visualization of the human erythrocyte membrane skeleton by freeze-etching

Abstract

The effect of fixatives on the membrane skeleton underlying the human erythrocyte membrane was examined by freeze-etching. An anastomosing fibrillar network was readily observed on the protoplasmic surface of the erythrocyte membrane treated with tannic acid. Such structure was much less defined in unfixed membrane or membrane fixed with glutaraldehyde or glutaraldehyde followed by osmium tetraoxide. Tannic acid caused a marked increase in diameter of the fibrillar components of the membrane skeleton and of the protoplasmic surface particles of inside-out vesicles prepared by alkali treatment but did not affect the size of intramembranous particles seen on fracture faces nor the appearance of exoplasmic surfaces. The improved visualization of the membrane skeleton after treatment with tannic acid resulted from interactions between tannic acid and exposed membrane proteins.