The rosetting antiglobulin test as a means of determining the D zygosity of human red cells

Abstract

The erythrocyte-antibody rosetting antiglobulin test was used to determine differences in the expression of various Rh antigens on homozygous and heterozygous Rh positive red blood cells. The rosetting antiglobulin test was able to detect the relative difference in expression of C, c, E and e on homo- and heterozygous red blood cells. Based on these findings, the test was used and found suitable for determining the genotypes of various D positive red blood cells.