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. 1984 Apr;47(4):677-80.
doi: 10.1128/aem.47.4.677-680.1984.

Microscale method for rapid isolation of covalently closed circular plasmid DNA from group N streptococci

P K Orberg, W E Sandine

Microscale method for rapid isolation of covalently closed circular plasmid DNA from group N streptococci

P K Orberg et al. Appl Environ Microbiol. 1984 Apr.

Abstract

A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolation procedure of McMaster et al. (Anal. Biochem. 109:47-54, 1980). The preparations obtained were highly enriched for covalently closed circular DNA, and the method was applicable to plasmids of at least 40 megadaltons. Centrifugation in CsCl-ethidium bromide density gradients was not required.

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