The receptor function of galactosyltransferase during cellular interactions

Abstract

The molecular mechanisms that underly cellular interactions during development are still poorly understood. There is reason to believe that complex glycoconjugates participate in cellular interactions by binding to specific cell surface receptors. One class of carbohydrate binding proteins that could serve as receptors during cellular interactions are the glycosyltransferases. Glycosyltransferases have been detected on a variety of cell surfaces, and evidence suggests that they may participate during cellular interactions by binding their specific carbohydrate substrates on adjacent cells or in extracellular matrix (see Refs. 1-4 for review). This review will focus on the receptor function of galactosyltransferase, in particular, during fertilization, embryonic cell adhesion and migration, limb bud morphogenesis, immune recognition and growth control. In many of these systems, the galactosyltransferase substrate has been characterized as a novel, large molecular weight glycoconjugate composed of repeating N-acetyllactosamine residues. The function of surface galactosyltransferase during cellular interactions has been examined with genetic and biochemical probes, including the T/t-complex morphogenetic mutants, enzyme inhibitors, enzyme modifiers, and competitive substrates. Collectively, these studies suggest that in the mouse, surface galactosyltransferase is under the genetic control of the T/t-complex, and participates in multiple cellular interactions during development by binding to its specific lactosaminoglycan substrate.