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. 1978 Feb 28;4(1):55-60.
doi: 10.1007/BF00775182.

Preparative density gradient centrifugation of RNA and DNA in cesium sulfate-urea mixture

S P Modak, M T Imaizumi, M Chappuis, K Scherrer

Preparative density gradient centrifugation of RNA and DNA in cesium sulfate-urea mixture

S P Modak et al. Mol Biol Rep. .

Abstract

A new procedure is described for a large scale separation and purification of unfixed DNA and RNA from a mixture of partially extracted nucleic acids and lysates of subcellular fractions by centrifugation to equilibrium in cesium sulfate-urea mixture. Optimum conditions are described for the separation and quantative recovery of both RNA and DNA in a pure form. The procedure allows determination of peak buoyant densities of 4-5s RNA, 7-11s mRNA and total cytoplasmic RNA. The procedure also allows fractionation of small molecular weight classes of cytoplasmic RNAs from the 18s and 28s rRNAs.

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