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. 1984 Jul-Aug;20(4):452-7.

[Purification of soybean trypsin inhibitor by an immunosorption method]

[Article in Russian]
  • PMID: 6433341

[Purification of soybean trypsin inhibitor by an immunosorption method]

[Article in Russian]
N V Fedurkina et al. Prikl Biokhim Mikrobiol. 1984 Jul-Aug.

Abstract

A technique for isolation of the trypsin inhibitor from soya beans (Kunitz inhibitor) was developed with affinity chromatography as a main step, the immobilized antibodies of the inhibitor being used as a sorbent. The inhibitor obtained was homogeneous according to the data of electrophoresis in PAAG and had the specific activity equal to that of an inhibitor preparation obtained by affinity chromatography on trypsin-sepharose.

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