Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1984 Sep;159(3):832-6.
doi: 10.1128/jb.159.3.832-836.1984.

Regulation of transcription of the Escherichia coli phosphoenolpyruvate carboxykinase locus: studies with pck-lacZ operon fusions

H Goldie
Comparative Study

Regulation of transcription of the Escherichia coli phosphoenolpyruvate carboxykinase locus: studies with pck-lacZ operon fusions

H Goldie. J Bacteriol. 1984 Sep.

Abstract

Mutants of Escherichia coli containing genetic fusions of lacZ to the pck (phosphoenolpyruvate carboxykinase) locus were isolated by using Mu d(lacZ Ampr) bacteriophage. Synthesis of beta-galactosidase in these strains is regulated by cyclic AMP and glucose (catabolite repression). Synthesis of beta-galactosidase by pck-lacZ fusions was induced in log-phase cells growing on gluconeogenic media, was repressed by glucose, and was also induced up to 100-fold at the onset of stationary phase in LB medium. This stationary-phase induction required cyclic AMP and some other unknown regulatory signal.

PubMed Disclaimer

References

    1. Science. 1970 Jul 24;169(3943):339-44 - PubMed
    1. J Biol Chem. 1972 Feb 25;247(4):1106-13 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2324-8 - PubMed
    1. J Bacteriol. 1977 Jan;129(1):246-53 - PubMed
    1. J Biol Chem. 1978 Jun 10;253(11):3945-51 - PubMed

Publication types

Substances

LinkOut - more resources