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. 1984 Oct;160(1):442-4.
doi: 10.1128/jb.160.1.442-444.1984.

Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases

F Kawamura, R H Doi

Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases

F Kawamura et al. J Bacteriol. 1984 Oct.

Abstract

A mutant strain of Bacillus subtilis carrying lesions in the structural genes for extracellular neutral (nprE) and serine (aprA) proteases was constructed by the gene conversion technique. This mutant had less than 4% of the extracellular protease activity of the wild type and sporulated normally, indicating that neither of these sporulation-associated proteases is essential for development.

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References

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