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. 1984 Oct 26;73(2):427-35.
doi: 10.1016/0022-1759(84)90417-4.

A simple and rapid solid phase enzyme-linked immunoadsorbence assay for screening monoclonal antibodies to poorly soluble proteins

G I Evan

A simple and rapid solid phase enzyme-linked immunoadsorbence assay for screening monoclonal antibodies to poorly soluble proteins

G I Evan. J Immunol Methods. .

Abstract

An ELISA is described in which protein antigens are fixed to microtitre wells. This assay is of particular use when antigens are soluble only in solutions containing SDS or other detergents, or else have been purified by preparative SDS polyacrylamide gel electrophoresis. Because the protein antigens are fixed to the microtitre wells, it is also possible to re-use antigen derivatised plates several times.

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