doi: 10.1016/0022-1759(84)90417-4.
A simple and rapid solid phase enzyme-linked immunoadsorbence assay for screening monoclonal antibodies to poorly soluble proteins
- PMID: 6436385
- DOI: 10.1016/0022-1759(84)90417-4
Item in Clipboard
A simple and rapid solid phase enzyme-linked immunoadsorbence assay for screening monoclonal antibodies to poorly soluble proteins
J Immunol Methods.
.
Abstract
An ELISA is described in which protein antigens are fixed to microtitre wells. This assay is of particular use when antigens are soluble only in solutions containing SDS or other detergents, or else have been purified by preparative SDS polyacrylamide gel electrophoresis. Because the protein antigens are fixed to the microtitre wells, it is also possible to re-use antigen derivatised plates several times.
Similar articles
-
Screening for monoclonal antibodies with covalently linked antigen.Dev Biol Stand. 1987;66:485-8. Dev Biol Stand. 1987. PMID: 3108055
-
A rapid solid-phase enzyme-linked binding assay for screening monoclonal antibodies to cell surface antigens.J Immunol Methods. 1981;44(2):125-33. doi: 10.1016/0022-1759(81)90340-9. J Immunol Methods. 1981. PMID: 6792282
-
A new ELISA plate based microtiter well assay for mycobacterial topoisomerase I for the direct screening of enzyme inhibitory monoclonal antibody supernatants.J Immunol Methods. 2010 May 31;357(1-2):26-32. doi: 10.1016/j.jim.2010.03.008. Epub 2010 Mar 17. J Immunol Methods. 2010. PMID: 20302873
-
A rapid and simple method for efficient coating of microtiter plates using low amounts of antigen in the presence of detergent.J Immunol Methods. 1986 Dec 4;95(1):117-22. doi: 10.1016/0022-1759(86)90325-x. J Immunol Methods. 1986. PMID: 3023494
-
Sodium dodecyl sulphate-treated proteins as ligands in ELISA.J Immunol Methods. 2002 Dec 1;270(1):19-26. doi: 10.1016/s0022-1759(02)00214-4. J Immunol Methods. 2002. PMID: 12379335
Cited by
-
The solid phase attachment of fungal hyphae in an ELISA to screen for antifungal antibodies.Mycopathologia. 1992 Nov;120(2):79-85. doi: 10.1007/BF00578292. Mycopathologia. 1992. PMID: 1480211
-
The N-Myc oncoprotein is associated in vivo with the phosphoprotein Max(p20/22) in human neuroblastoma cells.EMBO J. 1991 Dec;10(12):3703-12. doi: 10.1002/j.1460-2075.1991.tb04938.x. EMBO J. 1991. PMID: 1935896 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
