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. 1984 Dec;81(23):7525-8.
doi: 10.1073/pnas.81.23.7525.

Anti-mRNA: specific inhibition of translation of single mRNA molecules

S Pestka, B L Daugherty, V Jung, K Hotta, R K Pestka

Anti-mRNA: specific inhibition of translation of single mRNA molecules

S Pestka et al. Proc Natl Acad Sci U S A. 1984 Dec.

Abstract

A plasmid was constructed to generate RNA complementary to the beta-galactosidase mRNA under control of the phage lambda PL promoter. When this anti-mRNA was produced, synthesis of beta-galactosidase was dramatically inhibited (98%). Syntheses of galactoside permease and transacetylase, whose coding sequences are downstream of the beta-galactosidase coding region, are inhibited to a lesser degree, 80% and 55%, respectively. The generation of anti-mRNA that can be targeted to inhibit a single species of mRNA molecule within cells provides a potent mechanism by which specific transcripts can be translationally inactivated. This can be used to determine the function of proteins as well as to select cloned genes in a single rapid and convenient step.

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