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. 1984 Dec 28;125(3):1005-12.
doi: 10.1016/0006-291x(84)91383-4.

Rat hypothalamic growth hormone-releasing factor: isolation, sequence analysis and total synthesis

P Böhlen, W B Wehrenberg, F Esch, N Ling, P Brazeau, R Guillemin

Rat hypothalamic growth hormone-releasing factor: isolation, sequence analysis and total synthesis

P Böhlen et al. Biochem Biophys Res Commun. .

Abstract

Growth hormone-releasing factor (GRF) was isolated from acid extracts of approximately 35,000 rat hypothalami by means of immunoaffinity chromatography, gel filtration and two steps of reverse-phase HPLC. Amino acid analysis, gas-liquid phases sequencing and peptide mapping established that rat GRF is a 43 amino acid peptide with the amino acid sequence His-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly- Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu-Ile-Met-Asn-Arg-Gln-Gln-Gly- Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn-OH, confirming the primary structure reported earlier (Spiess et al Nature 303, 532 (1983).

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