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. 1980 Mar;28(3):255-62.
doi: 10.1177/28.3.6444434.

Determination of immunoreactive melatonin in the colon of the rat by immunocytochemistry

W R Holloway, L J Grota, G M Brown

Determination of immunoreactive melatonin in the colon of the rat by immunocytochemistry

W R Holloway et al. J Histochem Cytochem. 1980 Mar.

Abstract

Melatonin immunofluorescence in the rat colon was measured with a cadmium sulfide photometer in conjunction with an immunohistological double antibody procedure. The method proved to be very reliable with 5-10% intra- and interassay reliabilities. In addition, manipulation of the primary antibody concentration, the incubation time, and the nature of the first antibody produced predicted changes in fluorescence intensity. Treatment with p-chlorophenylalanine led to a marked reduction of melatonin-specific fluorescence.

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