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. 1980 Feb;77(2):799-803.
doi: 10.1073/pnas.77.2.799.

An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA

J Shlomai, A Kornberg

An Escherichia coli replication protein that recognizes a unique sequence within a hairpin region in phi X174 DNA

J Shlomai et al. Proc Natl Acad Sci U S A. 1980 Feb.

Abstract

Protein n', a prepriming DNA replication enzyme of Escherichia coli, is a phi X174 DNA-dependent ATPase. Restriction of phi X174 DNA have led to the identification of a 55-nucleotide fragment that carries the protein n' recognition sequence. Molecular hybridization and sequence analysis have located this sequence within the untranslated region between genes F and G, a map location analogous to that of the unique complementary strand origin of phage G4 DNA. Within the 55-nucleotide fragment is a sequence of 44 nucleotides that forms a stable hairpin structure. This duplex may be the signal for protein n' to initiate the prepriming events that led to the start of phi X174 complementary DNA strand replication.

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References

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