The postnatal development of the inferior oblique muscle of the cat. III. Fiber sizes and histochemical properties

Abstract

Transversal sections of the inferior oblique muscle from kittens of various ages and from adult cats were stained for myofibrillar ATP-ase at Ph 9.4 and 4.35, succinic dehydrogenase (SDH) and fat. The same muscles had previously been submitted to studies of contractional and fatigue properties. With ATPase the fibers could be differentiated into types I, II and II C. The percentages of each fiber type remained approximately the same from birth onwards. In the inner, global layer of the muscle, type I fibers showed a linear increase in size, but types II and IIC fibers an accelerated growth after age 20 weeks. In the outer, orbital layer, where no type I fibers were seen, type II and II C fibers showed the same growth pattern as in the global layer. The fiber content of SHD and fat was low at birth but increased after two weeks of age. Type I fibers were poor and type II C fibers rich in SDH and fat. Type II fibers showed varying amounts of these substances. Provided that type I fibers are slow, type II fast and type II C intermediate in speed of contraction, like in other muscles, the findings on fiber growth and SDH content seem to support the idea that slow, fatigue resistant components in eye muscles reach maturity earlier than fast components.