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. 1981;82(2):205-16.
doi: 10.1007/BF00286105.

Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes

F Scalenghe, E Turco, J E Edström, V Pirrotta, M Melli

Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes

F Scalenghe et al. Chromosoma. 1981.

Abstract

Fragments from section 3 of the salivary gland X chromosome of D. melanogaster were dissected with a micromanipulator. The DNA was extracted, cut and ligated to a lambda vector in a volume of a few nanoliters in an oil chamber monitored through a microscope. From about 10 pg of DNA we obtained 80 recombinant clones, a sample of which were analysed and shown to contain Drosophila DNA which hybridises in situ to the region of section 3 of the X chromosome. With this technique we can isolate clones from any desired region as small as 200 kb from the euchromatic arms of polytene chromosomes.

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