Ultrastructural cytochemistry of perinucleolar dense spots in heat-treated macroconidia of Neurospora crassa
- PMID: 6456905
Ultrastructural cytochemistry of perinucleolar dense spots in heat-treated macroconidia of Neurospora crassa
Abstract
Upon heat treatment at 46 degrees C of Neurospora macroconidia, numerous dense sports appear around the nucleolus but disappear completely 5 h after shift-down to normal temperature. Cytochemical reactions and high-resolution autoradiography after pulse-labeling with tritiated uridine indicate that these dense sports contain RNA (perhaps pre-rRNA), RNP and other proteins, but no DNA.
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