Metabolism of C19 steroids in vitro by the submaxillary salivary gland of mature and immature domestic pigs

Abstract

Homogenates of the submaxillary glands of immature and mature pigs were incubated with 3H-labelled C19 steroids which have been shown previously to be metabolized in vitro by the submaxillary gland of mature boars. Dehydroepiandrosterone was metabolized largely to androstenedione, 5 alpha-androstane-3,17-dione and androsterone, and to small amounts of testosterone, 5 alpha-dihydrotestosterone and 5 alpha-androstanediols. Testosterone yielded predominantly 5 alpha-androstane-3 alpha, 17 beta-diol with smaller amounts of other 5 alpha-reduced products, i.e. 5 alpha-dihydrotestosterone, 5 alpha-androstane-3 beta, 17 beta-diol, 5 alpha-androstane-3,17-dione and androsterone; 5 alpha-dihydrotestosterone and the two epimeric 5 alpha-androstane-3 alpha/3 beta, 17 beta-diols were interconverted. These and earlier results show that the porcine submaxillary gland has the capacity in vitro to metabolize selected C19 steroids in a way which is not related to either sexual maturity or sex of the animal; in this respect the findings support certain aspects of previous histochemical studies.