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. 1982 Mar;79(5):1403-7.
doi: 10.1073/pnas.79.5.1403.

Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells

D S Singer, R D Camerini-Otero, M L Satz, B Osborne, D Sachs, S Rudikoff

Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells

D S Singer et al. Proc Natl Acad Sci U S A. 1982 Mar.

Abstract

A porcine genomic clone encoding a major histocompatibility, (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC cDNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse transformant but not control cell lines in a complement-mediated cytotoxicity assay. Direct immunoprecipitation of radiolabeled cellular protein from transformed lines by pig alloantiserum results in the coprecipitation of swine MHC heavy chain and mouse beta 2-microglobulin, demonstrating the association of heterologous subunits of MHC antigens.

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