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Comparative Study
. 1978 Mar 1;169(3):465-70.
doi: 10.1042/bj1690465.

Glutathione S-transferase in human lymphoid cell lines and fractionated peripheral leucocytes

N Kaplowitz, C Spina, M Graham, J Kuhlenkamp
Comparative Study

Glutathione S-transferase in human lymphoid cell lines and fractionated peripheral leucocytes

N Kaplowitz et al. Biochem J. .

Abstract

Glutathione S-transferase activity was identified in cytosol from human lymphoid-cell lines and peripheral leucocytes (polymorphonuclear-leucocyte/monocyte and small-lymphocyte fractions) and compared with human liver enzyme. The findings of closely similar elution volume in gel filtration, substrate (1-chloro-2,4-dinitrobenzene) and inhibitory (probenecid) kinetics indicate that the liver, leucocyte and lymphoid-cell transferases are closely related. The interaction of reduced glutathione and 1-chloro-2,4-dinitrobenzene was shown to occur in intact-lymphoid-cell culture, to be linear with time and quantity of cells and to have kinetics similar to those of the enzyme reaction catalysed by cytosol.

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