Localization of glycosylated kappa-casein on thin sections of casein micelles by lectin-labelled gold markers

Abstract

The location of the glycosylated part of kappa-casein in bovine casein micelles was investigated using gold particles (6 nm in diameter) labelled with Ricinus communis lectin and Limulus polyphemus lectin. The pattern of marking of thin sections of micelles was similar with both lectins. Glycosylated kappa-casein was distributed uniformly throughout most micelles of all sizes. Peripheral location of glycosylated kappa-casein was observed only occasionally in some of the largest micelles. Quantitative data indicated that the concentration of the glycosylated protein was constant in micelles of increasing sizes. As larger micelles contain less total kappa-casein than smaller ones, these data indicated that a greater proportion of their kappa-casein is glycosylated. These results support models for casein micelle structure where kappa-casein is distributed throughout the micelles. They do not agree with "coat-core" structures.