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. 1984 Jun;95(6):1827-30.
doi: 10.1093/oxfordjournals.jbchem.a134797.

Immunological cross-reactivity between large and small (2'-5')oligoadenylate synthetases from pig cells

N Shimizu, Y Sokawa, J Sokawa
Free article

Immunological cross-reactivity between large and small (2'-5')oligoadenylate synthetases from pig cells

N Shimizu et al. J Biochem. 1984 Jun.
Free article

Abstract

Using glycerol gradient centrifugation, the molecular sizes of porcine (2'-5')oligoadenylate synthetases (2-5A synthetases) were estimated. The 2-5A synthetase purified from pig spleen was about 150 kDa, while the enzyme extracted from nuclei of Newcastle disease virus-infected pig epithelial cells (SK-h) was about 20-40 kDa. The nuclear 2-5A synthetase was selectively adsorbed to Protein A-Sepharose beads conjugated with anti-spleen 2-5A synthetase antibody. Thus, the smaller 2-5A synthetase in nuclei of pig cells shares a protein structure with the larger enzyme from pig spleen.

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