Structure of the N-asparagine-linked oligosaccharide units of human placental beta-glucocerebrosidase

Abstract

The N-asparagine-linked oligosaccharide chains of homogeneous human placental beta-glucocerebrosidase were released by hydrazinolysis, and their structures were analyzed. The sequence of sugars, linkage, and anomeric configuration of the glycosidic bonds were determined. Approximately 20% of the released sugar chain was of the typical high mannose type. The balance was of complex type with biantennary and triantennary structures. As a major component, the following trisialylated oligosaccharide was detected: (formula; see text).