doi: 10.1099/00221287-130-8-2023.
Studies on heterogeneous lipopolysaccharide fractions of Vibrio cholerae 569B
- PMID: 6470675
- DOI: 10.1099/00221287-130-8-2023
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Studies on heterogeneous lipopolysaccharide fractions of Vibrio cholerae 569B
J Gen Microbiol.
1984 Aug.
Abstract
By SDS-PAGE or gel filtration on Sephadex G-25, lipopolysaccharide (LPS) isolated from Vibrio cholerae 569B (Inaba) can be separated into two distinct fractions, one corresponding to smooth LPS and the other to rough LPS. Pulse-labelling of LPS with [14C]glucose showed that the rough form is synthesized first followed by the biosynthesis of the smooth form. A preferential release of the smooth LPS of V. cholerae 569B was also detected during normal growth of cells.
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