doi: 10.1007/BF00775148.
Chicken liver non-histone protein fraction repressing DNA transcription in vitro
- PMID: 6472255
- DOI: 10.1007/BF00775148
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Chicken liver non-histone protein fraction repressing DNA transcription in vitro
Mol Biol Rep.
1984 Jul.
Abstract
A chicken liver non-histone protein fraction repressing DNA transcription in vitro was isolated using ultrasound shearing followed by precipitation with divalent cations. It was characterized by SDS-polyacrylamide electrophoresis and isoelectric focusing. This inhibitory protein fraction showed strong affinity to DNA and represented non-histone proteins with main bands of mol. wt about 35000 and 56000 and isoelectric points about 5.7 and 6.2. The inhibitory non-histone protein fraction seems to repress DNA transcription by interacting with DNA template rather than with RNA polymerase.
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