Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Aug 24;12(16):6603-14.
doi: 10.1093/nar/12.16.6603.

Poly(pyrimidine) . poly(purine) synthetic DNAs containing 5-methylcytosine form stable triplexes at neutral pH

J S Lee, M L Woodsworth, L J Latimer, A R Morgan
Free PMC article

Poly(pyrimidine) . poly(purine) synthetic DNAs containing 5-methylcytosine form stable triplexes at neutral pH

J S Lee et al. Nucleic Acids Res. .
Free PMC article

Abstract

Poly(pyrimidine) . poly(purine) tracts have been discovered in the 5'-flanking regions of many eucaryotic genes. They may be involved in the regulation of expression since they can be mapped to the nuclease-sensitive sites of active chromatin. We have found that poly(pyrimidine) . poly(purine) DNAs which contain 5-methylcytosine (e.g. poly[d(Tm5C)] . poly[d(GA)]) will form a triplex at a pH below 8. In contrast, the unmethylated analogue, poly[d(TC)] . poly[d(GA)] only forms a triplex at pHs below 6. Synthetic DNAs containing repeating trinucleotides and poly[d(Um5C)] . poly[d(GA)] behave in a similar manner. Thus the stability of a triplex can be controlled by methylation of cytosine. This suggests a model for the regulation of expression based upon specific triplex formation on the 5'-side of eucaryotic genes.

PubMed Disclaimer

References

    1. J Biol Chem. 1970 Nov 10;245(21):5625-34 - PubMed
    1. Cell. 1981 Dec;27(3 Pt 2):413-5 - PubMed
    1. Biochemistry. 1974 Apr 9;13(8):1596-603 - PubMed
    1. Nucleic Acids Res. 1979 Jul 11;6(9):3073-91 - PubMed
    1. Nucleic Acids Res. 1979 Oct 10;7(3):547-69 - PubMed

Publication types

MeSH terms