Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Aug 1;221(3):593-9.
doi: 10.1042/bj2210593.

Regulatory effects of fatty acids on decarboxylation of leucine and 4-methyl-2-oxopentanoate in the perfused rat heart

D B Buxton, L L Barron, M K Taylor, M S Olson

Regulatory effects of fatty acids on decarboxylation of leucine and 4-methyl-2-oxopentanoate in the perfused rat heart

D B Buxton et al. Biochem J. .

Abstract

The regulatory effects of fatty acids on the oxidative decarboxylation of leucine and 4-methyl-2-oxopentanoate were investigated in the isolated rat heart. Infusion of the long-chain fatty acid palmitate resulted in both an inactivation of the branched-chain 2-oxo acid dehydrogenase and an inhibition of the measured metabolic flux through this enzyme complex. Pyruvate addition also caused both an inactivation and an inhibition of the flux through the complex. On the other hand, the medium-chain fatty acid octanoate caused an activation of and a stimulation of flux through the branched-chain 2-oxo acid dehydrogenase when the perfusion conditions before octanoate addition maintained the enzyme complex in its inactive state. When the enzyme complex was activated before octanoate infusion, this fatty acid caused a significant inhibition of the flux through the branched-chain 2-oxo acid dehydrogenase reaction. Inclusion of glucose in the perfusion medium prevented the octanoate-mediated activation of the branched-chain 2-oxo acid dehydrogenase.

PubMed Disclaimer

References

    1. Biochim Biophys Acta. 1965 Apr 12;100:295-8 - PubMed
    1. Biochem Biophys Res Commun. 1982 Aug 31;107(4):1497-503 - PubMed
    1. Am J Physiol. 1972 Dec;223(6):1376-83 - PubMed
    1. J Nutr. 1976 Aug;106(8):1079-88 - PubMed
    1. Biochim Biophys Acta. 1976 Jul 21;437(2):477-86 - PubMed

Publication types

MeSH terms