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. 1984 Aug 15;222(1):261-4.
doi: 10.1042/bj2220261.

Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase

R Mathur, A S Balasubramanian

Cobalt-ion chelate affinity chromatography for the purification of brain neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase

R Mathur et al. Biochem J. .

Abstract

A method is described for the purification of neutral alpha-D-mannosidase and its separation from acid alpha-D-mannosidase from monkey brain by utilizing Co2+ chelate affinity chromatography. The neutral enzyme, which selectively bound to the metal-ion chelate column, was elutable by Tris at pH 7.5 and gave over 80-fold purification in a single step with 100% recovery.

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