Regressive post-hatching development of acetylcholinesterase-positive neurons in the pineal organs of Coturnix coturnix japonica and Gallus gallus

Abstract

Distribution and number of acetylcholinesterase-positive neurons were studied in the Japanese quail and the domestic fowl during the post-hatching period by means of the acetylcholinesterase method. For comparison, the development of the catecholamine-containing (sympathetic) pinealopetal fibers of the domestic fowl was demonstrated with the use of the glyoxylic acid method. The number of acetylcholinesterase-positive ganglion cells in the pineal organs of both avian species decreased rapidly after hatching, with a concentration of these elements in the basal portion (stalk) of the pineal organ. In 3-day-old chickens, perivascular catecholamine-containing nerve fibers penetrate the antero-lateral walls of the pineal organ and are found exclusively in the interfollicular and perivascular tissues. In 13-day-old and adult fowl, these fibers increase in number and terminate not only in the interfollicular space but also in the neuroepithelial parenchyma of the pineal body. The ontogenetic regression of the sensory structures paralleled by an expanding sympathetic innervation in the pineal organ of a galliform species resembles somewhat the process of phylogenetic transformation leading from pineal sense organs to pineal glands.