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. 1984 Aug;48(2):454-5.
doi: 10.1128/aem.48.2.454-455.1984.

Purification of Giardia muris cysts by velocity sedimentation

J F Sauch

Purification of Giardia muris cysts by velocity sedimentation

J F Sauch. Appl Environ Microbiol. 1984 Aug.

Abstract

Giardia muris cysts were separated from fecal contaminants in primary isolates by unit gravity velocity sedimentation. Crude isolates obtained by centrifugation over 1.0 M sucrose were overlaid onto a Percoll density gradient, 1.01 to 1.03 g/ml. G. muris cysts were well separated from faster-sedimenting fecal debris and slower-sedimenting Spironucleus muris and bacteria in 1.5 h.

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