Retinal ganglion cells in two teleost species, Sebastiscus marmoratus and Navodon modestus

Abstract

Distribution patterns of ganglion cells in the retina were examined in Nissl-stained retinal whole mounts of Sebastiscus and Navodon. The existence of area centralis in the temporal retina in both species suggests binocular vision. In Navodon, another high density area was found in the nasal retina, and a dense band of ganglion cells was observed along the horizontal axis between the two high-density areas. There is an obvious trend for the ganglion cell size to increase as the density decreases. The total number of ganglion cells was estimated to be about 45 X 10(4) in Sebastiscus and 87 X 10(4) in Navodon, whereas the total number of optic nerve fibers was about 35 X 10(4) and 70 X 10(4), respectively. The retinal ganglion cells labeled with HRP were classified into six types according to such morphological characteristics as size, shape, and location of the soma as well as dendritic arborization pattern. Type I cells have a small round or oval soma in the ganglion cell layer and a small dendritic field in the inner plexiform layer. Type II cells are similar to type I cells, but the dendrites arborize more closely to the ganglion cell layer in the innermost region of the inner plexiform layer. Type III cells have a medium-sized round soma in the ganglion cell layer, and the dendrites extend in an extremely wide area in the inner plexiform layer with few branches. Type IV cells have a large soma which is located in the ganglion cell layer. Dendrites emanate from the soma in all directions, branching out several times within a rather small region in the innermost part of the inner plexiform layer. Type V cells have large somata of various shapes, usually dislocated to the inner plexiform or granular layer. The dendrites extend in every direction and occupy an extremely large area in the inner plexiform layer. Type VI cells have the largest somata, which are also dislocated to the inner plexiform or granular layer. Type VI cells have a characteristic triangular or fan-shaped dendritic field. Soma size and the axon diameter are intimately linked, that is, small somata of type I and II cells give off thin axons, and large somata of type V and VI give off thick axons.(ABSTRACT TRUNCATED AT 400 WORDS)