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. 1978 May;61(3):578-80.

Isolation and purification of moniliformin

M Steyn, P G Thiel, G C van Schalkwyk
  • PMID: 649549

Isolation and purification of moniliformin

M Steyn et al. J Assoc Off Anal Chem. 1978 May.

Abstract

A bulk purification procedure is described for moniliformin, a mycotoxin produced by Fusarium moniliforme. The method involves methanol extraction of suitably molded maize, aqueous extraction of the methanol-free residue, ion exchange chromatography with a NaCl concentration gradient, desalination, and crystallization. A pKa value of 1.70 as well as molar absorptivities of 19100 (lambda (H2O) max 229 nm), 5600 (lambda (H2O) max 260 nm), and 4700 (lambda (MeOH) max 260 nm) L/mole/cm are reported for moniliformin.

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