doi: 10.1016/0003-2697(84)90076-9.
Purification of glycogen phosphorylase from small quantities of mouse skeletal muscle
- PMID: 6496952
- DOI: 10.1016/0003-2697(84)90076-9
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Purification of glycogen phosphorylase from small quantities of mouse skeletal muscle
Anal Biochem.
1984 Sep.
Abstract
A new approach to the purification of skeletal muscle glycogen phosphorylase is described. The purification scheme is particularly suited to preparation of the enzyme from small amounts of tissue. A combination of dye-ligand chromatography and hydrophobic chromatography yields homogenous enzyme with good recoveries. The purification is rapid and may be completed in a working day.
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