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Comparative Study
. 1984 Dec;160(3):1181-3.
doi: 10.1128/jb.160.3.1181-1183.1984.

Simple, rapid, and quantitative release of periplasmic proteins by chloroform

G F Ames, C Prody, S Kustu
Comparative Study

Simple, rapid, and quantitative release of periplasmic proteins by chloroform

G F Ames et al. J Bacteriol. 1984 Dec.

Abstract

We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.

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