Development of a model system to evaluate methods for radiolabeling monoclonal antibodies

Abstract

We have developed a model system that can be used to evaluate methods for radiolabeling monoclonal antibodies. In this system, dinitrophenyl (DNP) is used as a model antigen, and HDP-1 (a monoclonal antibody specific for DNP) is used as a model monoclonal antibody. DNP-coupled agarose beads are injected into the femoral veins of rats, after which they localize in the animals' lungs. Radiolabeled antibody can then be injected and its biodistribution monitored. We describe here the development and initial testing of the model with radioiodinated antibody.