[Production of antibodies by hybridomas in a serum-free culture medium]

Abstract

Evidence is presented that hybridomas maintained in serum-free medium continue to secrete antibodies for some time. The dynamics of this secretion appears to be close to that in analogous cultures kept in the serum medium but apparently differs from that in analogous cultures grown in the balanced salt solution. The possibility of hybridomas to synthesize antibodies in protein free medium and not only to release those formerly synthesized is under discussion.