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. 1984 Sep;20(9):797-9.

Characterization of spiroplasma virus group 4 (SV4)

J Renaudin, M C Pascarel, M Garnier, P Carle, J M Bove
  • PMID: 6511355

Characterization of spiroplasma virus group 4 (SV4)

J Renaudin et al. Isr J Med Sci. 1984 Sep.

Abstract

Spiroplasma virus Group 4 was propagated, purified and characterized. The isometric capsid is made from one major protein of 60,000 Da and contains a circular single-stranded DNA molecule of 1.7 X 10(6) Da. The host-range of SV4 is limited to Subgroup I-2 spiroplasmas. The virions are released from the infected cells by a lytic process. Transfection of spiroplasmas of Subgroup I-2 by the viral DNA was demonstrated. A high efficiency of transfection was routinely obtained in the presence of polyethyleneglycol. These experiments indicate that spiroplasmas can absorb viral DNA, opening the way for cloning.

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