Caudal cuneate nucleus projection to the direct thalamic relay to motor cortex in cat: an electrophysiological and anatomical study

Abstract

The input to the border region between the ventrolateral nucleus (VL) and ventroposterolateral nucleus (VPL) of the thalamus, VL-VPL, was studied in cats using a combined electrophysiological and anatomical technique. Neurons within this border region receive somatic afferent input and project to a region of the motor cortex having similar receptive fields. In this study we asked the question whether neurons in the VL-VPL border receive input from the dorsal column nuclei (DCN). To answer this question we delivered intra-cortical microstimulation (ICMS) to the motor cortex while a second electrode inserted into the VL-VPL border, filled with a 20% solution of HRP dissolved in KCl, was used to record antidromically activated neurons. When an antidromically activated neuron was encountered and the neuron responded to natural peripheral stimulation, HRP was iontophoretically injected through the recording electrode. After a 48-72 h survival time, cats were sacrificed, and the brain tissue processed according to the method of Hardy and Heimer. Labeled cell bodies were found in the caudal cuneate nucleus (CCN) in all injected animals. These results suggested that neurons in CCN project to cells in VL-VPL which in turn project to the motor cortex.