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Comparative Study
. 1984 Dec 20;3(13):3069-73.
doi: 10.1002/j.1460-2075.1984.tb02260.x.

A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated

J Donson, B A Morris-Krsinich, P M Mullineaux, M I Boulton, J W Davies
Comparative Study

A putative primer for second-strand DNA synthesis of maize streak virus is virion-associated

J Donson et al. EMBO J. .

Abstract

We have isolated, from maize streak virus (MSV) preparations, a population of 'nested' DNA molecules. These molecules have ribonucleotides covalently linked to the DNA species' discrete 5' deoxyribonucleotide terminus. The major species has a DNA sequence of 80 nucleotides which is complementary to a region 5' of two hairpin structures on the MSV genome, almost exclusively in an intergenic region. These molecules have been used to prime the synthesis in vitro of a complementary strand to virion DNA, initiating this reaction at one site on the genome.

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References

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