An autoradiographic method for determining nutrient competition between leaf epiphytes and plant pathogens

Abstract

A method is described for the study of nutrient competition between leaf surface microorganisms and plant pathogenic fungi. Autoradiography was used to investigate the uptake of water-soluble 14C-labelled nutrients by applying a dry pre-formed layer of emulsion to the specimen. The method was adapted for use on both leaf and artificial surfaces. A photometric method was used for rapid examination of autoradiographs.