Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Mar;58(1):69-70.
doi: 10.1017/s0022149x00028078.

Separation of viable microfilariae free of blood cells on Percoll gradients

R Chandrashekar, U R Rao, G R Rajasekariah, D Subrahmanyam

Separation of viable microfilariae free of blood cells on Percoll gradients

R Chandrashekar et al. J Helminthol. 1984 Mar.

Abstract

A consistent and reproducible method is described for isolating pure populations of microfilariae of Litomosoides carinii, Brugia pahangi, B. malayi and Dipetalonema viteae, free of cells, from blood, by density gradient centrifugation on Percoll in 0.25 M sucrose. The recovery of the microfilariae was 85 to 97%.

PubMed Disclaimer

Publication types

MeSH terms