Developmentally regulated plasmalemmal glycoconjugates of the surface and neural ectoderm

Abstract

The plasmalemmal glycoconjugates of the ectoderm surrounding the rat embryo's caudal neuropore were mapped at the ultrastructural level, using various lectin probes. These included the agglutinins of wheat germ, soybean, Ricinus communis, Lotus tetragonolobus, and Canavalia ensiformis. Each lectin produced a characteristic binding pattern. Comparison of precursor cells of surface ectoderm, neural crest, and neural tube revealed that, even prior to neural tube formation, these three cell types can be distinguished by the sets of lectin receptors they express on their apical plasmalemma. The high density of lectin receptors found at the open neural groove level decreases dramatically during neurulation. Further changes in surface glycoconjugates must occur during neuronal differentiation because sprouting neurons exhibit yet another lectin binding pattern (K.H. Pfenninger, M.-F. Maylié-Pfenninger, L. B. Friedman, and P. Simkowitz, 1984, Dev. Biol. 106, 97-108). These results indicate that the commitment of ectodermal cells to diverging lineages (epidermis, neural crest, and tube) is reflected in their surface carbohydrates and occurs while they are still part of a continuous epithelial sheet. Furthermore, the plasmalemmal glycoconjugates of the ectoderm are developmentally regulated, and particularly dramatic changes in glycoconjugates expression are linked to neurulation.