Lectin labeling of sprouting neurons. III. Type-specific glycoconjugates on growth cones of different origin

Abstract

Ferritin conjugates of various lectins were used to determine the densities of surface carbohydrates on nerve growth cones produced by different classes of neuron. These neurons, from superior cervical and dorsal root ganglia, spinal cord, olfactory bulb, and cerebellum of the fetal rat, were grown as explant cultures, labeled with the probes, and then processed for quantitative electron microscopic analysis. It has been observed that each type of growth cone carries a characteristic set of lectin receptors on its surface, a "surface carbohydrate signature." Neurons derived from the neural tube generally exhibit lower levels of lectin binding sites on their growth cones compared with those derived from the neural crest. However, after neuraminidase treatment, lectin labeling is consistently dense for all growth cone types. These findings suggest (i) that neurons are programmed, possibly at the time of neurulation, to generate high- or low-sialic-acid patterns of surface carbohydrates on their growth cones according to their origin, and (ii) that the specific glycoconjugate pattern found for each type of neuron may be involved in selective cell-cell interactions during nervous system development.