Metabolism of prostaglandin E analogs in the guinea pig liver mitochondrial fraction

Abstract

Tritium-labeled 16,16-dimethyl-PGE2 and 9-methylene-PGE2 were incubated with the guinea pig liver mitochondrial fraction. The tetranor and dinor metabolites were obtained, a large portion of the latter contained a saturated carboxyl side chain. Also the beta-hydroxydinor metabolites, analogous to the beta oxidation of fatty acids, were identified. Such metabolites have not been reported earlier in connection with beta oxidation of prostaglandins or prostaglandin analogs. Furthermore, omega-hydroxylated analogs were incubated with the guinea pig liver mitochondrial fraction and were found to pass through beta oxidation to a certain extent.