Tumor-specific tRNA modifications in mouse plasmacytomas and other tumors

Abstract

RPC-5 chromatography has been used to analyze the aa-tRNA populations found in normal organs and in various tumors in experimental animals. The most extensively studied animal systems have been mineral-oil-induced mouse plasmacytomas and carcinogen-induced rat hepatomas. Certain aa-tRNA species appear to be tumor-specific, e.g., rat hepatoma phenylalanyl-tRNA1 and plasmacytoma asparaginyl-tRNAs2-4. In addition, one of the tumor-specific peaks of asparaginyl-tRNA can be found in normal livers of animals bearing plasmacytomas at a distant site. Many other significant quantitative and qualitative differences among histologically similar plasmacytomas and between normal tissues and plasmacytomas were observed in the chromatographic patterns of isoaccepting aa-tRNAs for 11 of 20 amino acids. Some of the qualitative differences in chromatographic patterns could be correlated with the tumorous nature of the tissue using computer analysis. The program utilized cluster analysis to compare the RPC-5 patterns of aa-tRNAs from 11 plasmacytomas and two normal tissues for each of the 20 amino acids. The variations in these chromatographic profiles are though to be caused by varying degrees of incomplete synthesis of some of the normally modified nucleosides in tRNAs.