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. 1978 May;35(5):878-80.
doi: 10.1128/aem.35.5.878-880.1978.

Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex

L J Moberg, H Sugiyama

Affinity chromatography purification of type A botulinum neurotoxin from crystalline toxic complex

L J Moberg et al. Appl Environ Microbiol. 1978 May.

Abstract

Type A botulinum neurotoxin was purified from toxic crystals by adsorption to p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. At pH 6.3, the toxic complex was held by the binding between the ligand and the hemagglutinin of the complex; the toxin is eluted selectively by dissociating the complex with buffer-saline of pH 7.9. The single-step affinity chromatography recovered 50 to 60% of applied toxicity as preparations of greater than 99% purity.

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