[Use of chromogenic substrates for estimation of indicators of kallikrein-kinin and blood coagulation systems in blood plasma of patients with nephrotic syndrome]

Abstract

A distinct increase of kallikrein content in blood plasma of patients with nephrotic syndrome as well as activation of thrombin simultaneously with a decrease in the prothrombin level were recorded in samples with synthetic chromogenic substrates S-2160 (Bz-Phe-Val-Arg-pNa), S-2238 (D-Phe-Pip-Arg-pNA) and S-2302 (D-Pro-Phe-Arg-pNA). Limited proteolysis of prothrombin by blood plasma kallikrein appears to be among the possible reasons of thrombin activation in the patients with nephrotic syndrome. Activity of plasmin, measured with S-2251 (D-Val-Leu-Lys-pNA) as a chromogenic substrate, was practically unlatered in blood plasma of these patients.